英文名字 Leukocyte RNA Purification Plus Kit
中文名称 白细胞RNA纯化大提试剂盒
供应商 Norgen Biotek
产品货号 NGB-21250
产品报价 ¥4158/50preps
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产品描述 Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. RNA isolated from blood can be used in various expression studies including those focusing on diseases. However, a major problem with blood RNA isolation is that a large portion of the RNA present is globin mRNA, which is found primarily in red blood cells. In fact, up to 70% of the mass of mRNA in whole blood total RNA is globin transcripts. Therefore, it is desirable to be able to remove the red blood cells from the sample and isolate only the RNA associated with the leukocytes, which will result in improved expression profiling and other applications by removing the masking effects of this abundant globin mRNA. Norgen’s Leukocyte RNA Purification Plus Kit can be used to isolate and purify total leukocyte RNA, including all small RNAs, from mammalian blood samples.
产品特点 Process up to 3 mL of blood and concentrate leukocyte RNA down to 10 to 20 礚; Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer; No phenol or chloroform extractions; Isolate total leukocyte RNA, including all small RNA species; Rapid and convenient spin-column format; Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more.
保存建议 溶液密封室温保存,未开封至少保存1年。RBC Lysis Buffer储存在4°C。具体保存情况收到产品后请按说明书存放。
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其他 Norgen Biotek目前的产品多达700余种,提供一流的样品制备试剂盒,应用于基因组和蛋白组等不同的领域。拥有超过二十个专利,作为一家成长型的公司,其产品主要覆盖包括各种标本类型的DNA,RNA和蛋白质的纯化富集,从活检样本(包括尿液,血浆,血清和血液)中纯化RNA和DNA,全系列的PCR试剂、缓冲液、分子量ladder和塑料过滤装置。此外,Norgen Biotek最近推出了100多款用于核酸诊断的试剂盒,包括从尿液、血浆和血清中诊断人类病原体的试剂盒,诊断食源性病原体的试剂盒和诊断植物病原体的试剂盒。这些试剂盒仅供研究使用,非常适合用于监测耐药病原体,流行病学研究,病原体现场监测和调查。Norgen Biotek还致力于为科研和临床试验提供高质量的分子诊断测试。

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